Engineering stability into Escherichia coli secreted Fabs leads to increased functional expression
نویسندگان
چکیده
منابع مشابه
Engineering stability into Escherichia coli secreted Fabs leads to increased functional expression.
The recombinant expression of immunoglobulin domains, Fabs and scFvs in particular, in Escherichia coli can vary significantly from antibody to antibody. We hypothesized that poor Fab expression is often linked to poor intrinsic stability. To investigate this further, we applied a novel approach for stabilizing a poorly expressing anti-tetanus toxoid human Fab with a predisposition for being mi...
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ژورنال
عنوان ژورنال: Protein Engineering Design and Selection
سال: 2006
ISSN: 1741-0126,1741-0134
DOI: 10.1093/protein/gzl016